the effect of in planta tiba and l-proline in cell culture media on somatic embryogenesis of sugar beet (beta vulgaris l.)

the effect of in planta tiba and proline on in vitro seedlings and cell culture of sugar beet was investigated. sterilized seeds were grown in vitro on ½ strength ms medium supplemented with 0 or 3 mg/l tiba. calli obtained on young leaves cultured on ms medium containing 1 mg/l bap, were used for the intiation of cell suspension cultures using ms basal composition supplemented with 0 or 50 m m proline. aliquats of 1 ml from cell suspension culture were inoculated onto the first somatic embryo induction ms medium containing tiba 0.5 mg/l, bap 1.0 mg/l and proline at 0 or 50 mm. after three weeks of culture, embryogenic calli were transferred to the second embryo induction medium supplemented with naa and bap at 0.2 and 0.5 mg/l respectively. the frequency of somatic embryos of calli obtained from in planta tiba together with proline treatments on average was 20 which was higher than that of the other treatments.